As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications.
Main Features:
Up to 6 fluorescence detection channels allowing multiplex PCR.
Effectively reduce multi-color crosstalk and edge effect, no ROX correction required to reduce sample and reagent use
Innovative scanning method and time-resolved signal separation technology to improve detection sensitivity
Unique edge temperature compensation technology to minimize "edge effect"
User-friendly software
Innovative technology with long-lastig LED light provides reliability results
| Temperature control system | |
| Sample capacity | 96 |
| Reaction volume | 10-50 μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0° C/s |
| Heating temperature range | 4 – 100 °C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
| Temperature gradient setting range | 30–100°C |
| Temperature gradient difference setting range | 1 – 36°C |
| Detection system | |
| Excitation light source | 4/6 monochrome high efficiency LEDs |
| Detection device | PMT |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection wavelength range | 455-650nm/510-715nm |
| Fluorescent channels | 4/5 channels |
| Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex QPCR |
| Dynamic range | 10 orders of magnitude copies |